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Transgenic/Knock-out Mouse Shared Resource

Mission

Genetically engineered mouse models (GEMMs) have become indispensable tools in cancer research, with their utility ranging from studies of the basic biology of tumorigenesis and progression to the creation of genetically accurate tumor models for the evaluation of novel therapeutic approaches. The objectives of the L.T. Christian III Transgenic/Knockout Mouse Shared Resource are to:

  1. Facilitate the development of new GEMMs (including transgenic, knockout, conditional knockout and knock-in mice) using both conventional and CRISPR-Cas9-based technologies
  2. Provide a full range of support services to facilitate the use of these models, including breeding colony management, mouse genotyping, mouse line rederivation, embryo and sperm cryopreservation, and cryorecovery
  3. Offer expert consultation on the development and use of GEMMs 

Who we are

The Transgenic/Knockout Mouse Shared Resource is directed by Jolene Windle. Greg Campbell. the resource manager, has experience with all aspects of GEMM production and cryopreservation, and he oversees the IVIS Spectrum imager located in the MMRB vivarium. Mark Subler brings expertise in all aspects of GEMM-related molecular biology and is responsible for the resource's genetic services, including transgene/targeting vector/CRISPR-Cas9 reagent design, synthesis of CRISPR-Cas9 reagents, and genotyping. Pamela Watters has been involved in mouse husbandry for many years, she and is responsible for breeding colony management.

Please contact Dr. Windle for any inquiries. 

 Citation

All publications that include results, services or products generated by the VCU Massey Cancer Center Shared Resources must include the following acknowledgement in the manuscript:

"Services in support of the research project were provided by the VCU Massey Cancer Center Transgenic/Knockout Mouse Core, supported, in part, with funding from NIH-NCI Cancer Center Support Grant P30 CA016059."

PubMed Central (PMC) ID numbers are required for those publications that use CCSG-supported shared resources. All of Massey Cancer Center's shared resources are supported by the CCSG.