Transgenic/Knock-out Mouse Shared Resource
Genetically engineered mouse models (GEMMs) have become indispensable tools in cancer research, with their utility ranging from studies of the basic biology of tumorigenesis and progression to the creation of genetically accurate tumor models for the evaluation of novel therapeutic approaches. The objectives of the L.T. Christian III Transgenic/Knockout Mouse Shared Resource are to:
- Facilitate the development of new GEMMs (including transgenic, knockout, conditional knockout and knock-in mice) using both conventional and CRISPR-Cas9-based technologies
- Provide a full range of support services to facilitate the use of these models, including breeding colony management, mouse genotyping, mouse line rederivation, embryo and sperm cryopreservation, and cryorecovery
- Offer expert consultation on the development and use of GEMMs
Who we are
The Transgenic/Knockout Mouse Shared Resource is directed by Jolene Windle. Greg Campbell. the resource manager, has experience with all aspects of GEMM production and cryopreservation, and he oversees the IVIS Spectrum imager located in the MMRB vivarium. Mark Subler brings expertise in all aspects of GEMM-related molecular biology and is responsible for the resource's genetic services, including transgene/targeting vector/CRISPR-Cas9 reagent design, synthesis of CRISPR-Cas9 reagents, and genotyping. Pamela Watters has been involved in mouse husbandry for many years, she and is responsible for breeding colony management.
Please contact Dr. Windle for any inquiries.
- Jolene Windle, Ph.D.
- Greg Campbell
- Pamela Watters
Breeding colony manager
Mark Subler, Ph.D.
Genetic services manager