VCU Massey Cancer Center


Suggested grant language

Investigators contemplating the inclusion of core laboratory services in a pending grant application are strongly encouraged to consult with the faculty core director in order to craft grant language tailored to a particular application, and to develop a suitable budget for the proposed services. However, as a starting place, the following language may be used as a template:

 "The VCU Massey Cancer Center Flow Cytometry Shared Resource was established in 1976, and has been constantly modernized since that time. It currently occupies over 1500 sq ft in the Kontos Medical Science Building and the Molecular Medicine Research Building, and provides a comprehensive instrumentation suite and technical services in support of both cell sorting and analysis on a fee-for-service basis. The core currently features six primary instruments. Cell sorting is conducted using two multi-laser BD Aria II sorters. One of the Aria units is enclosed in a Baker BioProtect Hood, enabling the sorting of human and biohazardous tissues.  Sorting capabilities include up to 9 color simultaneous detection (including UV and violet laser capability) and up to 4 simultaneous sorts per Aria II.  The core supports three analyzers, a BD FACSCantoII, a BD LSRFortessaX20 , and an Amnis ImageStreamX MarkII.  In addition to flow instrumentation, the core also maintains a Reichert surface plasmon resonance (SPR) instrument for determination of protein interaction constants. The director, Daniel H. Conrad, has over 30 years of experience in flow cytometry. The laboratory manager and head technician, Julie Farnsworth, is highly trained in flow cytometry has been with the core for more than 15 years. The resource provides a wide range of services, including instrument training, routine fluorescence analysis, and development of innovative custom analysis and sorting protocols. Walk-up instrument time is also available to trained users. This core maintains a stable base of instrumentation, expertise, and trained personnel and currently provides services and training for multiple users in over 60 research groups from 21 departments at VCU. The VCU Massey Cancer Center Flow Cytometry Shared Resource is supported, in part, by funding from the NIH-NCI Cancer Center Support Grant P30 CA016059."